The double stranded regions of RNA are formed by base-pairing between residues in a region complementary to the region of the editing site.
Additional stabilisation is achieved by interactions between adjacent residues in the flanking region and hydrophobic interactions.
It is calculated as the average sequence distance between residues that form native contacts in the folded protein divided by the total length of the protein.
This causes OmpT to selectively cleave peptides between two basic (positively-charged) residues.
One of the issues with either of your suggested methods is that these may not discriminate between salts and other residues in water.
Studies suggest that the interactions between the excited state product and nearby residues can force the oxyluciferin into an even higher energy form, which results in the emission of green light.
Polar interactions are formed between the HMG-moiety and residues that are located in the cis loop of the enzyme.
A BLAST search reveals a putative receptor binding site exists between residues 75-124.
Important stabilizing hydrogen bonding between various residues on the AgTx2 and the subunits of the channel hold the toxin-channel complex together.
A proline rich region is also predicted to lie between residues 161-170.