The bottom strand is numbered in the same way from the 5' end, but with primes on the numbers.
Each oligonucleotide is designed to be either part of the top or bottom strand of the target sequence.
As they ran out into the sunlight again he saw that Job and Dedan had a hold on the bottom strand of the fence.
But Flair fled in terror, sliding under the bottom strand like an eel wriggling free.
We could look under the bottom strand of wire and see the big oak tree about five hundred yards away.
By convention, the top strand is written from the 5' end to the 3' end; thus, the bottom strand is written 3' to 5'.
He lay down on the ground and scooted beneath the bottom strand of barbed wire.
On the bottom strand, it's on the right (the 5' end is to the left).
The acid began to eat away at the edge of her tunic, the bottom strands of her curly hair.
Holding it by the wooden butt, he laid it across the bottom strand of fence wire.