"atmosphere" in inglés with examples - Collocation dictionary inglés
- Cell culture NIH 3T3 fibroblasts were grown in DME medium containing 1000 mg/liter glucose, supplemented with 10% FCS, 4 mM L-glutamine, penicillin (100 IU/ml), and streptomycin (100 μg/ml), at 37 C in a humidified atmosphere of 5% CO 2 in air.
- Monolayers were grown in a humidified atmosphere of 95% air/5% CO 2 at 37 C.
- The cells were incubated for 14 hours in a humidified atmosphere with 5% CO 2 in air at 37 C.
- Cells were maintained in a humidified atmosphere of 5% CO 2 at 37 C. Adapters and primers The sequences of the adapters and primers were modified from reference [ 6 ] for better priming efficiency.
- The procedure was repeated twice, and the supernatants were pooled and cultured in one 100 mm plate and incubated at 37 C in a 5% CO 2 humidified atmosphere.
- Cells were cultured in Dulbecco's modified Eagle medium (DMEM), 10% fetal bovine serum (FBS), and 40 μg/ml gentamicin at 37 C in a humidified atmosphere of 5% CO 2 .
- Cell proliferation assay (MTT assay and cell counting) MCF-7 cells were grown in culture plates (tissue culture grade, 12 wells, flat bottom) in a final volume of 1 ml serum-containing culture medium per well, in a humidified atmosphere at 37 C and 5% CO 2 for 3 days.
- Freshly isolated GC were plated in the basic serum-supplemented culture media on collagen-coated 24-well (500 μl/well) or 6-well (2 ml/well) culture dishes, and incubated at 37 C in a humidified atmosphere of 5% CO 2 and air.
- Cultures were maintained in humidified atmosphere of 5% CO 2 at 37 C. For binding studies, tsA201 cells were plated at a density of 5 x 10 6cells/75 cm 2and grown for 9 hours prior to transfection.
- These cell lines were grown at 37 C in a humidified atmosphere containing 5% CO 2 .
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