"vector" in inglés with examples - Collocation dictionary inglés
- Second antibody and subsequent incubations were done according to the specifications of a Vectastain ABC Kit (Vector Laboratories) and colour was visualized with 3,3'-diaminobenzidine.
- Immunohistochemical stainings were performed using Vector ABC kits (Vector Laboratories) following the manufacturer's recommendations and as described [ 25].
- Other reagents, including the secondary antibody, detection kits, and chromogen kits, were purchased from Vector Laboratories, Inc (Burlingame, CA).
- Immunohistochemical staining was performed using an indirect immunoperoxidase detection protocol (Vectastain Elite kit; Vector Laboratories) following treatment of sections with hyaluronidase to improve antibody penetration.
- Anti-human spectrin antibodies (NCL-SPEC1) were obtained from Vector Laboratories.
- Antigen-antibody complexes were detected by a DABNi detection kit according to the supplier's manual (Vector Laboratories, Inc., Burlingame, CA), giving the substrate dark blue color.
- The samples were washed extensively in PBS and then incubated with biotinylated secondary antibodies (Vector Laboratories, Burlingame, CA).
- DNA was counterstained with Vectashield (Vector Laboratories) containing 1-5 μg/ml 4,6-diamidino-2-phenylindole (DAPI).
- "Photoprobe biotin", Vector Laboratories, Inc., www.vectorlabs.com.
- Diaminobenzidine (DAB substrate kit for peroxidase, Vector Laboratories, Burlingame, CA) was used as the final chromogen.
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