"vector" in inglés with examples - Collocation dictionary inglés
- Following transfection, however, both the sense and antisense probes would be expected to recognize vector DNA.
- Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules.
- Typically, this is done by cleaving the vector DNA and foreign DNA with the same restriction enzyme, for example EcoRI.
- Next, the vector DNA can be taken up by a host organism- commonly a population of Escherichia coli or yeast- with each cell containing only one vector molecule.
- These fragments are then joined with vector DNA to produce recombinant DNA molecules.
- This prevents recircularization of linearized vector DNA and improves the yield of vector containing the appropriate insert.
- Therefore, the long DNA molecules that compose an organism's genome must be cleaved into fragments that can be inserted into the vector DNA.
- The linear vector DNA already has the topoisomerase enzyme covalently attached to both of its strands' free 3' ends.
- The concept is important in molecular biology, especially in cloning or when subcloning inserts DNA into vector DNA.
- An expression cassette is a part of a vector DNA used for cloning and transformation.
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