"wash" in inglés with examples - Collocation dictionary inglés
- The cells were then treated with 1 μM ionomycin for 30 minutes, after which time they were either fixed immediately or washed extensively with PBS and incubated for 6 hours in medium lacking the drug ("washout").
- Membranes were stripped at 50 C in, washed extensively with TBST, blocked and hybridized as described above using a mouse anti-actin primary antibody at 1:1000 and HRP-conjugated goat anti-mouse IgG secondary antiserum as described above.
- Subsequently, slides were gently agitated in 0.2% SDS (no salt) overnight at 42 C, washed extensively with water, scanned to ensure that the dye was completely removed, restained with Vistra Green and rescanned.
- Rib cages were transferred to phosphate buffered saline (PBS) and washed extensively.
- The cells were briefly hemolyzed, washed extensively in DMEM containing 10% FBS, filtered through a 100 μM sterile nylon filter, counted by hemacytometer, and suspended in complete medium.
- After extensively washing with the same buffer, the column was eluted with buffer 3 plus 0.15 M (NH 4 )2 SO 4.
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