bacterial artificial chromosome

Sequencing in Medicago truncatula is based on bacterial artificial chromosomes.

Sequencing of this region was done with the aid of bacterial artificial chromosome clones.

One popular way of studying EBV in vitro is to use bacterial artificial chromosomes.

RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells.

Insert size of up to 350 kb can be cloned in bacterial artificial chromosome (BAC).

With TAR cloning, the desired regions could be isolated from the genome directly, rather than through construction and screening of new bacterial artificial chromosome libraries.

To package even more DNA into a vector, bacterial artificial chromosomes or yeast artificial chromosomes can be used.

Bioengineers have created F plasmids that can contain inserted foreign DNA; this is called a bacterial artificial chromosome.

Bacterial artificial chromosome (BAC)

Yeast artificial chromosomes and bacterial artificial chromosomes were created before human artificial chromosomes, which first appeared in 1997.

Sirion uses technology involving bacterial artificial chromosomes (BAC), which allows the company to design and construct viral vectors from scratch.

We obtained a bacterial artificial chromosome (BAC) clone (686I6) containing genomic sequence of the APBA2 transcriptional unit.

Bacterial artificial chromosomes (BACs) are circular DNA molecules, usually about 7kb in length, that are capable of holding inserts up to 300kb in size.

BAC (Bacterial Artificial Chromosome) arrays were historically the first microarray platform to be used for DNA copy number analysis.

Knockout is accomplished through a combination of techniques, beginning in the test tube with a plasmid, a bacterial artificial chromosome or other DNA construct, and proceeding to cell culture.

To clone longer lengths of DNA, lambda phage with lysogeny genes deleted, cosmids, bacterial artificial chromosomes or yeast artificial chromosomes could be used.

In simulations, Velvet can produce contigs up to 50-kb N50 length using prokaryotic data and 3-kb N50 in mammalian bacterial artificial chromosomes (BACs).

Bacterial Artificial Chromosome (BAC)-end sequencing: This is another method used in oncogenomics, which identifies chromosomal breakpoints in a high-resolution manner.

P1 artificial chromosomes (PACs) have features of both P1 vectors and Bacterial Artificial Chromosomes (BACs).

These pieces were then ligated into a type of vector known as "bacterial artificial chromosomes", or BACs, which are derived from bacterial chromosomes which have been genetically engineered.

Due to these issues, the Human Genome Project ultimately abandoned the use of YACs and switched to bacterial artificial chromosomes, where the incidence of these artifacts is very low.

In conclusion, the results demonstrate that our novel selection system is useful for isolation of large centromeric regions that are poorly clonable in bacterial artificial chromosome vectors [ 22 ] .

Characterization of the TAR isolates has shown that the gap sequences to be toxic to E. coli , which may explain why those regions were not found in bacterial artificial chromosome libraries.

If the DNA to be cloned is exceptionally large (hundreds of thousands to millions of base pairs), then a bacterial artificial chromosome or yeast artificial chromosome vector is often chosen.

A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli.