cell pellet

Do not suck red cell pellets into the suction bottle.

Cell pellets obtained from the swabs were used to isolate DNA.

Carefully remove the supernatant (without disturbing the cell pellet) and transfer to a second Sterilin tube.

Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets.

Immunohistochemistry assay Cell pellets were first embedded to paraffin block and sections cut and mounted onto microscope slides.

Fixed cell pellets were washed twice with cacodylate buffer and twice with 0.5 M ammonium chloride.

Supernatants were collected while the cell pellets were suspended in 0.5 ml 80% ethanol, and both were stored at -70 C until assayed.

Resuspend cell pellet in 0.5 mL sterile Milli-que (MQ) water or equivalent.

The cell pellet was fixed by adding 2.5% glutraldehyde for 4 h and the pellet was washed in sodium cacodylate buffer.

Frozen tumor tissues or cell pellets were utilized for DNA and/or RNA isolation by standard methods.

The cell pellet was washed once in cold phosphate-buffered saline and subsequently resuspended in hypotonic lysis buffer (10 mM Tris.

Northern analysis Cell pellets were lyzed and RNA extracted using the RNeasy method (Qiagen), following the manufacturer's instructions.

Adjust cell density by either diluting with sterile TSB or centrifuging and resuspending the cell pellet in a smaller volume of sterile broth.

The cell pellets were resuspended in sodium dodecylsulfate (SDS)-sample buffer at 1 x 10 4cells per μl and then sonicated to shear nucleic acids.

After incubation the tubes were centrifuged at 300 g and cell pellet was washed gently in Hank's balanced salt solution (HBSS) at least thrice.

LAESI is a novel ionization source for mass spectrometry (MS) that has been used to perform MS imaging of plants, tissues, cell pellets, and even single cells.

The DNA bank prepares high-quality, high-molecular-weight DNA from the cell pellet using a modification of the salting-out procedure of Miller et al. [ 40 ] .

After centrifugation, the cell pellet was resuspended with staining buffer (prechilled to 4 C) consisting of phosphate-buffered saline solution with 0.2% BSA and 1 μg/ml sodium azide.

The cell pellets were washed three times in phosphate buffered saline (PBS)(2 ml) to remove any adherent drug before being resuspended in 1 ml 0.05 M phosphate buffer.

Bacterial cells are harvested via centrifugation and the resulting cell pellet lysed either by physical means or by means of detergents and enzymes such as lysozyme or any combination of these.

The resulting cell suspensions were centrifuged in Eppendorf tubes and cell pellets were fixed for 5 min at room temperature using a 1% glutaraldehyde in 0.2M sodium cacodylate, pH 7.2.

Intracellular 5-ASA and Ac-ASA were measured by resuspending the cell pellet in 1 ml 0.05 M phosphate buffer, and disrupting it ultrasonically by six, 5 second bursts using an ultrasonicator (MSE).

The cell suspension was then over-layed onto 15 ml of lymphocyte-separation medium (LSM; Organon Teknica Corp., Durham, NC) for separation of a monocyte/lymphocyte interphase and a granulocyte/red blood cell pellet.

The cell pellets were stored at -70 C. Cell lysis was monitored by fluorescent microscopy and aliquots of column fractions were assayed in the wells of 96 well microtiter plates for green fluorescence using a Molecular Dynamics Storm Phosphorlmager.

Briefly, cells were transferred into the wells of round-bottom 96-well plates, the plates were centrifuged at 200 g for 30 seconds, the supernatant removed by shaking, and the cell pellets resuspended in 50 μl of staining buffer containing saturating concentration of anti-MHCII-FITC.