coverslip

(vii) Take off the top coverslip which served as a lid.

This reaction is then covered with a coverslip and left for two to four days in a humid chamber at 40 C.

This can also happen on inverted microscopes because the coverslip is below the slide.

Mix the solutions and cover with a coverslip.

R values were measured in 40 l droplets of each solution on a glass coverslip.

The agarose is gelled at 4 C and the coverslip removed.

The cells first lose adhesion to the coverslip, retract and roundup.

Mounting a coverslip on a specimen on a microscopic slide.

The depression containing the coverslip was placed perpendicularly to the flow of perfusate.

Reduce the volume of the drops, remove the slide from the oil, drain it slightly and apply a glass coverslip.

The thickness of the coverslip is crucially important for high-resolution microscopy.

Mounting medium and a coverslip were applied.

After staining, microscope slides are put in xylene prior to mounting with a coverslip.

Immediately, place the aminosilane-treated coverslip on top of the acrylamide gel and allow to polymerize for 45 minutes.

Finally, the sample must be properly mounted under a coverslip to minimize any other optical effect that could complicate the interpretation of the color seen.

As the coverslip is lowered onto the slide the molten agarose spreads to form a thin layer.

In our experiments, the coverslip and the cells represent the media with high and low refractive indices, respectively.

(ii) Wash the coverslip off using a wash bottle containing de-ionized water.

Remove the slide and the coverslip and rinse off the staining mixture using de-ionized water.

HEp-2 cells were transferred to tissue culture treated microplates with 15mm wells, each containing a sterile 13mm glass coverslip.

A glass cover slip is held at an angle and the mono-suspension applied to the point of contact between the coverslip and the slide.

It is also used after sections have been stained to make them hydrophobic so that a coverslip may be applied with a resin in solvent.

(vii) Seal around the coverslip with rubber solution (e.g. Holdtite).

The cells were grown in antibiotic free medium for two days to give a semiconfluent monolayer of cells on each coverslip.

The raised edges of the hemocytometer hold the coverslip 0.1 mm off the marked grid, giving each square a defined volume .