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Carbonate content was measured manometrically by reaction with dilute HCl.
Non-specific disorders of motility were manometrically classified with Castell criteria.
These reference gases are themselves calibrated against specific standard gases whose CO2 concentrations are determined manometrically.
The tips of both catheters were then placed 5 cm above the manometrically determined lower oesophageal sphincter and the probes taped to the nares, cheek, and neck.
In the standard test the oesophageal pH was monitored 5 cm above the manometrically defined lower sphincter during four provocations (deep breathing Valsalva manoeuvre and cough, for three times, and Trendelenburg position for three minutes), when a fall to pH<4 was considered to indicate reflux.