Galiano asked, having listened to ES cells 101.
"Which led him to an interest in ES cells."
ES cells with the correct targeted gene are then injected into a diploid host mouse blastocyst.
These factors are extremely important for the efficiency of deriving ES cells.
Generation of human ES cells is more difficult and faces ethical issues.
Without the initial deacetylation on Day-1, the ES cells cannot go through the differentiation.
"ES cells could become a valuable commodity."
God knows how he thought the ES cells fit in.
ES cells do not display contact inhibition and are not seen to enter Go cell cycle arrest.
Using targeted ES cells also provides the potential of expressing lethal transgenes.