The events that led to the Massacre center on Francis, Duke of Guise.

On 10 July 1541, Christina married Francis, Duke of Bar.

The sovereignty of the provinces was first offered to Francis, Duke of Anjou.

Having been converted to the Huguenot cause, he determined to kill Francis, Duke of Guise.

The marriage never took place, as Isabella was wed to Francis, Duke of Cádiz.

He went into medical practice and became physician to Francis, Duke of Anjou.

In February 1563 Francis, Duke of Guise was assassinated.

Children from the marriage with Francis, Duke of Guise (1519-1563):

This was also the case with Francis, Duke of Cádiz.

Her last courtship was with Francis, Duke of Anjou, 22 years her junior.