Gorgon is referenced in The Avengers: Earth's Mightiest Heroes episode "Widow's Sting".

The Kree Sentries appear in The Avengers: Earth's Mightiest Heroes episode "459."

The New Avengers are featured in The Avengers: Earth's Mightiest Heroes episode named after the team.

Bi-Beast has appeared in The Avengers: Earth's Mightiest Heroes episode "Hulk vs the World".

The Sleeper appears in The Avengers: Earth's Mightiest Heroes episode "Winter Soldier."

Rhodes appears in The Avengers: Earth's Mightiest Heroes episode "Iron Man is Born!"

Jocasta appears in The Avengers: Earth's Mightiest Heroes episode "Ultron Unlimited."

Annihilus appears in The Avengers: Earth's Mightiest Heroes episode, "Assault on 42."

The Destroyer appears in The Avengers: Earth's Mightiest Heroes episode "Powerless."

The Microverse appeared in The Avengers: Earth's Mightiest Heroes episode "Yellowjacket."