To achieve maximum purity in a single step, affinity purification can be performed, using the antigen to provide exquisite specificity for the antibody.
Tandem affinity purification (TAP) is a technique for studying protein-protein interactions.
After 2 successive affinity purifications, the chance for contaminants to be retained in the eluate reduces significantly.
Cell fractionation and affinity purification Bacterial cells were fractionated as previously described [ 18 ] .
Tandem affinity purification (TAP) method allows high throughput identification of protein interactions.
Another use for the procedure is the affinity purification of antibodies from blood serum.
This allows for affinity purification of proteins that are not well-behaved when fused to protein tags.
Chitin may be employed for affinity purification of recombinant protein.
Polyhistidine-tags are often used for affinity purification of genetically modified proteins.
Membrane receptors may be isolated from cell membranes by complex extraction procedures using solvents, detergents, and/or affinity purification.