Specific amplification products detected on Southern blots with a radiolabelled internal probe were subcloned and sequenced.

Direct DNA sequencing of the relevant amplification products in both patients was used to characterize each mutation.

The amplification products from various sources were also cloned and sequenced, the first determination of new alleles by PCR.

The resulting high concentration of fluorescence in each single-molecule amplification product is easily visible as a distinct bright spot when viewed with a fluorescence microscope.

Primer pair T-11 and T-12 corresponds to transcript-1 and produce an amplification product of 579 base pairs.

The analysis of the genotypes of the sample usually involves sizing of the amplification products by gel electrophoresis.

The appearance of an amplification product therefore indicates the genotype.

Standard curves for each amplification product were generated from 10-fold dilutions of pooled cDNA to determine primer efficiency.

Various detection and capture steps are involved in each approach to assess the amplification product, or amplicon.

However, similar experiments do not yield amplification products from the cyst nematodes we tested.