After the amplification reaction, several-hundredfold replication of the DNA circle has occurred, and labeled complementary oligonucleotide probes highlight the product.

To answer these questions and to define an optimal protocol for T7 based linear amplification, we carried out a series of amplification reactions under different conditions using total RNA isolated from primary human breast carcinomas.

This technique involves the use of minisatellite core sequence as a primer, singly, in the amplification reactions.

In general, amplification reactions with an increase of less than 300 units were scored as negative.

The amplification reaction consisted of the following: 50 C for 2 min, denaturation at 95 C for 10 minutes, and 40 thermal cycles of 95 C for 15 s and 60 C for 1 min.

As the product of an amplification reaction, "Amplicon" is used interchangeably with common laboratory terms, such as PCR product.

Three independent RT-PCR products were cloned from separate amplification reactions and sequenced.

Any sort of amplification reaction or catalysis reaction obliterates easy quantification but allows presence to be detected very sensitively.

Increasing numbers of primer annealing events happen along the amplification reaction.

The amplification reaction initiates when multiple primer hexamers anneal to the template.