The appropriate antibody is first incubated with Protein A or Protein G-agarose and allowed to bind.

Proteins were transferred to PVDF membranes and analyzed with either anti-T7 or SKI antibodies and the appropriate secondary antibodies.

Cells were then washed twice with PBS and incubated for 30 min at 4 C with the appropriate FITC-conjugated second antibody.

By choosing appropriate antibodies, the differentiation of leukemic cells can be accurately determined.

Each section was preincubated with 2% bovine serum albumin for 1 hour, then incubated overnight at 4 C with the appropriate primary antibody at 1:500 dilution.

Membranes were probed with the appropriate antibodies.

The immune system responds with appropriate and effective antibodies and killer T cells.

However, the signal has to be associated with the presence of antibody or antigen, which is why the enzyme has to be linked to an appropriate antibody.

Immunogenicity results from the fact that appropriate parental antibodies are obtained from rat and mice.

In addition, finding the appropriate antibody could require extensive screening of many antibodies by western blotting prior to beginning RPMA analysis.