Standardized bacterial suspensions containing Fusobacterium nucleatum, Campylobacter rectus and Peptostreptococcus micros were inoculated into the upper chambers.

Stable TFX expression would be beneficial under field conditions when the biological control agent is inoculated on seeds or on roots dipped in a bacterial suspension prior to planting.

A 5 μl drop of bacterial suspension was placed on the wound and allowed to air dry.

In the latter, much larger volumes of bacterial suspension are grown from which a maxi-prep can be performed.

Equal volumes of the two bacterial suspensions were mixed to produce a suspension containing a 1:1 ratio of recombinant-to-control bacteria.

Equal volumes of bacterial and cell suspensions were mixed and shaken gently at room temperature for 30 minutes.

The uptake was initiated by adding equal volume of radiolabelled 55Fe siderophore to the bacterial suspension and shaken at 300 rpm.

When needed, the bacterial and phage suspensions were serially diluted in 0.85% saline or LB Glucose before plating.

In microbiology, McFarland standards are used as a reference to adjust the turbidity of bacterial suspensions so that the number of bacteria will be within a given range.

If the bacterial suspension is too turbid, it can be diluted with more diluent.