The cleavage reactions were incubated at 37 C for 5 min and then divided into three aliquots.

The products were analyzed as described for the cleavage reactions.

A small steady stream of sulfuric acid is added to catalyse the cleavage reaction.

This is the cleavage reaction observed with the full-length intron and several length variants with a relative low rate.

These reactions are both ring opening reactions and cleavage reactions.

Th IN cleavage reaction was performed basically as described previously (22).

Effect of NCp7 on the IN cleavage reaction.

Rather, the cleavage reaction is simply an isomerization that consists of rearrangement of the linking phosphodiester bond.

This cleavage reaction generates the unusual aldose sugar erythrose-4-phosphate.

This intermediate is isoenergetic, meaning that the forward cleavage reaction and the backward religation reaction are both energetically equal.