Toxins isolated-form culture supernatants were inactivated once exposed to oxygen after being pre-incubated with cholesterol.

On the 2 ndday, culture supernatants were removed from each well and fresh medium was added.

On the 4 thday, culture supernatants were collected and tested for p24 antigen by ELISA.

In the early 1970s, Dr. Kishimoto discovered the activity inducing antibody production in culture supernatants of T cells.

However, when immunoabsorbed against each of the antisera alone, the effect on neurotoxicity of the culture supernatants was not significant (not shown).

The recombinant genes were transfected into Chinese hamster ovary cells and the recombinant antibody harvested from culture supernatant.

Analysis of culture supernatants for the presence of SpeB (Fig.

The culture supernatant can yield 1 to 60 g/ml of monoclonal antibody, which is maintained at -20 C or lower until required.

Briefly, aliquots of culture supernatants (100 μL) were mixed with either 1 μg of purified human plasminogen or buffer.

Briefly, 50 μL of culture supernatant with or without 0.1 μM dithiothreitol, was added to the wells of a microtiter plate.