Panel (D ): copy numbers are determined by scanning on a PhosphorImager as described in Material and Methods.

We transformed these plasmids into wild type animals as described in Materials and Methods and screened for transgenic lines.

Performance comparisons Computational comparisons of the two approaches were performed using the C-code implementations developed as described in Methods.

To quantify these differences the quantitative analyses described in Materials and Methods were carried out, and the results are summarized in Figure 2.

Therefore, we employed a gel-extraction method (33) and renatured the eluted protein as described in Materials and Methods.

The above experiment was extended to determine the sequence of both subunits essential for this interaction as described in the Materials and Methods.

Deletions were cloned as described in Materials and Methods and transfected into F9 cells by electroporation.

In vitro transcription reactions were performed as described in Materials and Methods.

The employed protein fractions were prepared as described in Materials and Methods.

The time-lapse video was created from the captured images as described in Material and Methods, with a time compression of 3.5 min (i.e., 7 images) per second.