Most enzymes that break down proteins, including those that allow the body to digest food, do not need energy to work.
Elevated enzymes have been associated with later clinical outcomes such as higher risk of death, subsequent MI and need for repeat revascularization procedures.
It occurs when an enzyme needed to break down certain very long-chain fatty acids is missing or not working properly.
However, the enzymes would still need to be replaced after a few years.
Furthermore, intracellular enzymes need not be extracted prior to the reaction; they may be used directly.
Indeed, if the developing human fetus provides some of its own nutrition, digestive enzymes may need to be synthesised and secreted during interuterine life.
But the enzyme is normally dormant because the water in those soils keeps them free of oxygen, which the enzyme needs to function.
This enzyme needs Ca for activity.
But the enzymes need zinc to function properly.
Some enzymes do not need any additional components to show full activity.