This is further enlarged to show the spine in more detail and the boxed region from which the fluorescence measurements were obtained.
"The majority of fluorescence measurements are now made using modulated fluorometers with the leaf poised in a known state."
The noise in fluorescence measurements has an impact on the precision of qPCR.
Excitation at 524 nm and emission at 589 nm was used for fluorescence measurements.
To maximize the robustness and quantitative accuracy of the array, comparative fluorescence measurements are made, using an internal standard for each protein to be assayed.
Aliquots from this final suspension were subjected to centrifugation and washed twice immediately prior to fluorescence measurement.
The number of voltage steps in fluorescence measurements was kept to a minimum to avoid rundown of the Ca 2+transient due to photobleaching.
Spatially resolved instruments are typically automated microscopes, and temporal resolution still requires some form of fluorescence measurement in most cases.
Originally, strand dissociation was observed using UV absorbance measurements, but techniques based on fluorescence measurements are now the most common approach.
Therefore the use of conventional, steady-state fluorescence measurement presents serious limitations in assay sensitivity.