A new product was a fluorescent molecular tag for immunodiagnostic assays.
This requires the use of specific antibodies to marker molecules with fluorescent tags recognized by the instrument.
For starters, people got rid of the radioactivity by replacing it with a fluorescent tag.
If we think of things in terms of information content, we've got four fluorescent tags that are stable and easily distinguishable.
Man-made GFP became commonly used as a fluorescent tag to show which cells or tissues express specific genes.
In live cell imaging, fluorescent tags enable movements of proteins and their interactions to be monitored.
They fused two cells labeled with different membrane-bound fluorescent tags and watched as the two dye populations mixed.
The most common analysis involves labeling proteins with fluorescent tags, and finally changes in cell phenotype are measured using automated image analysis.
Through the use of fluorescent tags with different absorption and emission maxima, it is possible to measure several different cell components in parallel.
Although this approach can need a lot of material, its sensitivity can be increased by labelling the substrates/products with a radioactive or fluorescent tag.