Gap filling The gap is filled by DNA polymerase using free nucleotides and the ends of the probe are ligated by ligase, resulting in a fully circularized probe.

In an RNA world, different forms of RNA compete with each other for free nucleotides and are subject to natural selection.

These free nucleotides are added to an exposed 3'-hydroxyl group on the last incorporated nucleotide.

When free nucleotides are washed over this chip, light is produced as ATP is generated when nucleotides join with their complementary base pairs.

The DNA molecule uncoils from one end, the opposite strands separate, the bonds between opposite bases break, and the free nucleotides find their opposite nucleotide on each strand.

Some naturally competent bacteria also secrete nucleases into their surroundings, and all bacteria can take up the free nucleotides these nucleases generate from environmental DNA.

The L protein then transcribes five mRNA strands and a positive strand of RNA all from the original negative strand RNA using free nucleotides in the cytoplasm.

The absorbance is a natural property of DNA, RNA, free nucleotides, proteins and some amino acids and many other compounds as well.

Nucleic acids (precisely the single, free nucleotides, not those bound in a DNA/RNA strand) has an extremely short lifetime due to a fast internal conversion.

DNA polymerase can add free nucleotides only to the 3' end of the template strand.