The viral pellet was resuspended in fresh medium by gentle pipetting.

On the 2 ndday, culture supernatants were removed from each well and fresh medium was added.

Recover the eggs by inverting the mesh over a second Petri dish and passing fresh medium through it.

At this point the culture has to be diluted with fresh medium if it is intended for further use.

The medium was replaced with fresh medium lacking G-418.

The fragments shown are typical of those produced by toothpick transfer of a mature fiber into fresh medium.

Control samples were rinsed in DPBS, drained, and fresh medium was added.

The original DNA content (one copy) is restored when replanting the culture into a fresh medium.

To the futurists, cinema was an ideal art form, being a fresh medium, and able to be manipulated by speed, special effects and editing.

The cells were then washed to remove any unattached virus and fresh medium was added.