The Coast Guard said that from 100,000 to 250,000 gallons had spilled.
For the first two days after the accident, the Coast Guard estimated that only 50,000 gallons of oil had spilled.
More than 37 million gallons of crude oil spilled into the water and caused massive environmental damage.
Some 260,000 gallons of fuel have now spilled into the turquoise waters around the Galapagos since last week.
More than a million gallons of petroleum products have spilled this year into the harbor, where water quality has recently been improving.
Town officials say 2.5 million gallons of sewage spilled into the river.
Initially, the company said 10,000 gallons of oil had spilled, but on Sunday revised the estimate to 30,000 to 40,000.
Judging from how much oil was left in the two oil-containing compartments, officials estimated that 4 million gallons had spilled.
Altogether 100,000 gallons have spilled, officials said, but much was burned up on top of the water and much more confined by booms.
As much as 75 million gallons of sewage spilled, threatening a wildlife refuge.