Another important consideration in microarray experiments is the time allowed for the hybridization reaction.

The hybridization reaction was carried out overnight at 58 C with 50 μl of hybridization mix on each section.

Probe synthesis, hybridization conditions, and wash regimen were performed as described above for hybridization reactions.

It uses molecular "barcodes" and microscopic imaging to detect and count up to several hundred unique transcripts in one hybridization reaction.

Slides were then treated, and the hybridization reaction was carried out as described at [ 42].

Most hybridization reactions could be analyzed after 2 hours, although in several instances up to 8 hours of hybridization time was required.

We conclude that both DAP modification and an increased hybridization time can affect the hybridization reaction but in very different ways.

For example, it is used in standard DNA extraction and hybridization reactions in molecular biology.

The hybridization reactions were processed and scanned according to standard Affymetrix protocols.

RS conducted the hybridization reactions and DNA sequencing.