He married Cicely Baker in 1555 and had seven children, including his heir Robert.

They had nine children, four daughters and five sons, including his heir George Osborne.

They had two sons, including his heir Charles Stanhope.

They had three sons, including his heir Richard Needham.

By Alice, Guy had three children, including his heir and successor, Thomas.

They had 7 children, including his heir, James Ludovic.

They had six children, including his heir Louis William.

She had five children by the king including his heir, later Henry III.

By her he had three sons including his heir, Richard.

He had four sons, including his heir Charles, by his first wife, and one son by his second wife.