Essentially identical results were observed with 10 individual clones and one, clone 5, was the example used here.
The total population of individual clones obtained in a molecular cloning experiment is often termed a DNA library.
These variants can then be ligated into an expression vector, individual clones can be created, and the encoded protein variants can be expressed.
DNA was extracted from individual clones.
After the third round of enrichment, individual clones were isolated and analyzed.
Microsynteny is generally maintained at the scale of individual fosmid clones, and the few rearrangement breakpoints, transposable elements and gene transpositions can readily be identified.
The cDNAs used for EST generation are typically individual clones from a cDNA library.
An average of eight individual clones were analyzed for every OR cloned.
Retests of individual clones revealed that 90% were bona fide activators.
Minipreps of DNA from individual clones were sequenced using the T7 priming site of the cloning vector.