Thus, the only way to make AAV is to repeatedly insert DNA for the virus into a new set of producer cells.
Directly inserting eukaryotic DNA into bacteria would not work because it carries introns, so would not translate successfully using the bacterial ribosomes.
In essence it consists of inserting foreign DNA - which contains the genetic information necessary to confer the target characteristic - into a vector.
Using gene transfer techniques, researchers in his lab inserted DNA from human bladder tumor cells into normal animal cells.
Transfection and transformation are also common ways to insert DNA into a cell.
In 1963, Tong inserted DNA of a male carp into the egg of a female carp and became the first to successfully clone a fish.
But in order to facilitate axonal repair, I inserted DNA from a species of diatom.
Three microbiologists at the university reported they have successfully inserted foreign DNA into mosquito eggs.
In contrast, we've known how to work with silkworms for centuries and, in recent years, we've developed the ability to insert DNA into their genome.