To do this, a scale, or calibration curve, must be constructed using several known concentrations for each compound of interest.

You start by creating a graph using known concentrations of Chlorine.

Sometimes an internal standard is added at a known concentration directly to an analytical sample to aid in quantitation.

The complex has been referred to as "the greatest known concentration of mechanical power in the ancient world".

Within twenty years, the find proved to be the richest known concentration of copper in the world.

A set of samples with known concentrations are spotted alongside the sample.

After a short period (usually less than a minute) to allow binding, add a known concentration of the conjugate.

With up to 700 ppm, the seeds of woody plants have the highest known concentrations.

As a result, the cave also holds the record for the largest known concentration of mammals in the world.

There were also no known concentrations of enemy foot soldiers, though that information was far less certain than the other.