The δ-endotoxin genes are usually located on large plasmids.
Comparative sequence analysis of these large plasmids with pXO2 could determine if the observed sequence conservation was located on these plasmids.
In practice the best achievable result may be around 2-4x10 cfu/μg for a small plasmid like pUC19, and considerably lower for large plasmids.
Recently, proteins similar to tubulin and FtsZ have been discovered in large plasmids found in Bacillus species.
This genetic and catabolic diversity is not only due to the large bacterial chromosome, but also to the presence of three large linear plasmids.
The larger plasmids carry other genes as well as the colicin operon.
The genes required for mycolactone biosynthesis form a contiguous 110-kb cluster on a large plasmid.
Soon after successfully cloning two pSC101 plasmids together to create one large plasmid, they published the results describing the experiment, in 1973.
Plasmids and bacteriophages are usually replicated as single replicons, but large plasmids in Gram-negative bacteria have been shown to carry several replicons.
All strains isolated from foals and the majority of human, cattle and pig isolates contain a large plasmid.