When antibodies were used to locate proteins of the TIP group, the signal was always confined to tonoplast membrane fractions [ 72].
The membrane fraction was lysed in Triton X-100 lysis buffer.
Thus, CD98 association with integrin has another level of specificity beyond simply co-localizing in light membrane fractions.
This cleavage occurs in the chloroplast membrane fraction.
Sometimes a particular membrane fraction can be isolated first, such as isolating mitochondria from cells before purifying a protein located in a mitochondrial membrane.
Peripheral and integral membrane fractions can be extracted from the remaining membrane fraction.
The mean level of AA in the membrane fractions of the three tissues was not consistently changed.
Insig2 was also found to localize to the mitochondria/heavy membrane fraction and associate with conformationally changed Bax.
The majority of wild type CD98 heavy chain and control mutant (C330S) are localized to the light membrane fraction (55-60%, Fig.
These results are consistent with the CD98 light chain targeting the CD98 heavy chain to light membrane fractions.