Lack of glial differentiation, as demonstrated by negative staining for glial fibrillary acidic protein (GFAP), may confer a favourable prognosis.
Myeloperoxidase staining is still important in the diagnosis of myeloid sarcoma, contrasting with the negative staining of lymphomas, which can otherwise have a similar appearance.
In addition, the patients with negative staining had no involvement of lymph nodes.
Sample preparations involve depositing specimens onto a coated TEM grid and negative staining with an electron-opaque liquid.
The virus is about 30 nm in size, and can be observed with transmission electron microscopy using negative staining.
For bright field microscopy, negative staining is typically performed using a black ink fluid such as nigrosin.
The F particle is large and can be seen in the transmission electron microscope by negative staining.
Note: negative staining is a mild technique that may not destroy the microorganisms, and is therefore unsuitable for studying pathogens.
Furthermore, negative staining with nigrosin can reveal some microorganisms that cannot be stained by regular methods.
AH protein was shown by positive staining with anti-Β 2 -microglobulin antibody and by negative staining with other antibodies.