The protected fragments of the probes were resolved in 5% denaturing polyacrylamide gel followed by blotting to a positively-charged nylon membrane.

For ribotyping, the gels were then transferred to nylon membranes by means of vacublotting.

After that, about a half a teaspoon of the sample is diluted with another chemical, turning it pink, and then it is filtered onto a nylon membrane.

A nylon membrane with a positive charge is the most effective for use in northern blotting since the negatively charged nucleic acids have a high affinity for them.

This was transferred to a nylon membrane and exposed to a probe.

Southern analysis was performed with 30 g of human genomic DNA on nylon membrane as described (36).

When Citroën designed their Hydractive 3 suspension they re-designed the spheres with new nylon membranes, which greatly slow the rate of deflation.

Samples were electrophoresed on 0.8% agarose gels and the size fractionated products transferred to nylon membranes using standard procedures.

The primary antibody (chicken IgY, 62.5 μg/ml) was diluted in blocking solution and incubated overnight at 4 C with the nylon membrane.

Genomic DNA was isolated as described, subjected to restriction enzyme digestion, and immobilized on a positively charged nylon membrane.