Twice daily rinsing with Listerine, in conjunction with normal oral hygiene (brushing and flossing), significantly reduced plaque over six months.
After washing, cells were subsequently incubated with a 1:100 dilution of fluorescein isothiocyanate-labeld goat anti-mouse antibodies (Kirkegaard and Perry Laboratory) and rinsed twice in PBS.
At various times, cultures were rinsed twice with Tyrode's balanced salt solution, fixed with 1% glutaraldehyde (v/v) in Tyrode's for 15 min, rinsed twice with deionized water, and air-dried.
Rinse twice with tap water.
And the baby's clothes and bedding should be washed with a mild, unscented detergent like Arm & Hammer and rinsed twice.
Rinse twice to remove all traces of the cleaning product.
Rinse twice with deionized water.
Now rinse twice with cold water.
The sections were then stained with Luxol Fast Blue (in 95% ethanol) for 13 minutes at room temperature, rinsed twice with 100% ethanol and cleared overnight in a third 100% ethanol rinse.
Drain, rinse twice and set aside.