In the second step, two sequence specific primers hybridise to each border of the DNA template.

Methylation is determined by the ability of the specific primer to achieve amplification.

Then denatured DNA is mixed with a specific primer.

However, specifying a larger mismatch value may make it more difficult to find such specific primers.

The oligonucleotide pool presented in this study was composed of a random 25-mer flanked by specific primers.

To insert a mutation into a DNA sequence, a specific primer is designed.

This step is known as the annealing step and is primer specific.

A primer specific for human β-actin was added to a parallel reaction to serve as an internal standard.

Transmission electron microscopy or PCR (using specific primers) is needed for speciation.

Strong conservation of the sequences upstream and downstream from the repeats allowed the design of very specific primers that generate extremely reproducible results.