During prophase, two aster-covered centrosomes migrate to opposite sides of the nucleus in preparation of mitotic spindle formation.

It is required for the recruitment of several different proteins important to the spindle formation.

Amitosis is a stochastic process where unlike in mitosis, there is no spindle formation to segregate chromosomes during nuclear division.

During cell division Demecolcine inhibits mitosis at metaphase by inhibiting spindle formation.

The first step in spindle formation is the nucleation of microtubules by microtubule-organizing centers, which then grow in all directions.

Multipolar spindles are spindle formations characteristic of cancer cells.

A possible causes of multipolar spindle formation involve regulation of protein kinase family known as Aurora kinase.

Attachment of microtubules to chromosomes is mediated by kinetochores, which actively monitor spindle formation and prevent premature anaphase onset.

The completion of spindle formation is a crucial transition point in the cell cycle called the spindle assembly checkpoint.

Instead, the nuclear envelope acts as a microtubule organizing center (MTOC) for spindle formation during preprophase.