Consistently, however, L. sicculus prefers clear water with low substrate concentrations.

This method would be used in many streams with varying levels of substrate concentration, temperature, vegetation, pollution, and invasive species.

Oxidation or reduction depletes the substrate concentration, resulting in diffusion from either layer.

The substrate concentration midway between these two limiting cases is denoted by K.

Positive cooperativity makes enzymes much more sensitive to [S] and their activities can show large changes over a narrow range of substrate concentration.

Because of this the product mixture can be tuned by reaction conditions, such as gas pressure and substrate concentration.

In substrate inhibition there is a progressive decrease in activity at high substrate concentrations.

The Michaelis constant is the substrate concentration at which the reaction rate is half of .

Thus it holds if the enzyme concentration is much less than the substrate concentration.

It has been shown that high substrate concentrations as well as a wide variety of functional groups are tolerated.