A representative dot plot of unstimulated and stimulated cells is shown in Figure 1.
One study was able to simultaneously measure the fold-change in phosphorylation state of 127 proteins between unstimulated and EphrinB1-stimulated cells.
It is predominantly expressed in activated T cells, but can also be found at low levels in unstimulated cells.
In unstimulated cell arrestins are localized in the cytoplasm in this basal "inactive" conformation.
Human spry2 expression is localized to the microtubules in unstimulated cells.
These receptor subunits are expressed in activated monocytes and in unstimulated epithelial cells.
Only those cells from ulcerative colitis, however, increased their activity significantly (p<0.05) above that of unstimulated cells.
No phosphorylated tyrosine residues were detected on Syk in unstimulated or control treated cells (lanes 1,4, and 5).
However, even when more protein was present in the immunoprecipitate, little if any phosphotyrosine was detected in unstimulated cells (lanes 13 and 15).
In unstimulated cells, the mdm2 protein binds to the N-terminal transactivation domain of p53 and targets it for ubiquitin-dependent degradation [ 6, 7].