The deduced amino acid sequence can be saved in various formats and searched against protein databases using BLAST.

A variety of tools are available for searching the SNP database, allowing search by genotype, method, population, submitter, markers and sequence similarity using BLAST.

Orthologs were found all the way back to the scarlet sea anemone and homologs were found in bacteria and Archaea using BLAST.

The basic level of annotation is using BLAST for finding similarities, and then annotating genomes based on that.

Sequence identity and similarity were determined using BLAST with the reference human sequence as the query.

The below table shows some orthologs found using BLAST.

We then carried out additional sequence analyses of these genes using BLAST, Pfam [ 21] and COG to confirm our prediction.

The below table shows some, but not all, orthologs which were found using BLAST.

To determine if it might be a BDNF 5' exon it was compared with all known sequences using BLAST.

We used BLAST to search annotated repeats against the rice repeat database, using an E-value cutoff of 10 -8.