"gentle" in inglés with examples - Collocation dictionary inglés
- After blocking, a dilute solution of primary antibody (generally between 0.5 and 5 micrograms/mL) is incubated with the membrane under gentle agitation.
- A product reminiscent of yogurt would have been produced, which, through gentle agitation and the separation of curds from whey would have resulted in the production of cheese; the cheese being essentially a concentration of the major milk protein, casein, and milk fat.
- The mix was incubated for two hours in the cold room with gentle agitation, the beads were pelleted by spinning briefly on a bench top Eppendorf centrifuge, washed four times with PBB containing no BSA, and the pellet was dried under vacuum for twenty minutes.
- Each mixture was incubated at 4 C for one hour with gentle agitation.
- Using the anti-capillary forceps, hold the dried grid face-down on the surface of the ethanolic PTA for 1 min with gentle agitation, drain on the surface, transfer to the surface of 95% ethanol to rinse for 15-30 sec, drain on filter paper and allow to air-dry.
- The beads were re-suspended with 50 μl of the same buffer, and added into the fragment-probe mix and incubated at room temperature for 30 minutes with constant gentle agitation.
- The muscularis mucosae is composed of several thin layers of smooth muscle fibers oriented in different ways which keep the mucosal surface and underlying glands in a constant state of gentle agitation to expel contents of glandular crypts and enhance contact between epithelium and the contents of the lumen.
- The slide was incubated in a static incubator at 42 C for 12-16 h, and washed by placing in a 250 ml solution of 2X SSC and 0.1% SDS at 42 C for 5 minutes with gentle agitation provided by rotation of a magnetic stir bar.
- The slide was transferred quickly to a solution of 0.1X SSC, and 0.1% SDS, incubated for 10 minutes at room temperature with gentle agitation, and washed 5 additional times in 0.1X SSC for 1 min.
- To generate cells that constitutively expressed human IL-1β, skin was first harvested from a euthanized Wistar rat (Charles River Laboratories, Wilmington, MA, USA), minced with a scalpel, and digested for 2 hours at 37 C under gentle agitation with 0.2% clostridial collagenase.
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